Figure 4.

The sav1 mutation results in decreased acid phosphatase secretion. Acid phosphatase secretion was induced by depletion of phosphate as described in Materials and Methods and is shown corrected for cell density and normalized to wild-type levels. □, SAV1; ■, sav1; ▩, sav1::SAV1; all strains are serotype A. The mean and SE values plotted were compiled from seven independent experiments performed in duplicate for the SAV1 and sav1 strains, and four independent experiments performed in duplicate for the sav1::SAV1 strain. Statistically significant differences were observed between the sav1 mutant and wild-type strains (p < 0.001 at both temperatures) and between the sav1 mutant and complemented strains (p = 0.003 at RT; p < 0.001 at 30°C). There was no significant difference between wild-type and complemented strains (p = 0.19 for RT; p = 0.21 for 30°C).