Figure 7.

sav1 cells contain more total vesicles than SAV1 cells, with or without latrunculin B (LatB), but in LatB conditions the vesicles are no longer polarized to the bud. For each strain, cells were shifted to 30°C for 3 h concurrent with LatB or ethanol (control) treatment and examined by EM. Vesicles present in sections of 60 budding cells were counted, and the average numbers of vesicles per section in the mother and bud were plotted. ▩, vesicles in a mother cell; ■, vesicles in a bud/daughter cell. Mean and SE are plotted. The fraction of vesicles observed in the bud decreased significantly in each strain upon LatB treatment [45.6 to 33.6% (p = 0.02) for SAV1; 66.2 to 28.8% (p < 0.001 for sav1 cells)].