Figure 8.

savl cells accumulate vesicles that contain GXM. Strains were grown in YPD at RT for 6 h and then shifted to 30°C for 3 h (for serotype A strains) or for 1.5 h (for serotype D strains). Ultrathin sections were labeled with the anti-GXM mAb 3C2 and 12-nm gold-conjugated anti-mouse antibody as described in Materials and Methods, and images of buds are shown. Similar results were obtained using the anti-GXM mAb 2H1 (unpublished data). (A) Serotype A strains; scale bar, 0.4 μm. (B) The serotype D sav1 strain; scale bar, 0.2 μm. Arrowheads, labeled vesicles; Cap, capsule; other abbreviations as in Figure 5. Note that when ∼100-nm vesicles are cut into 80-nm sections, in many cases only part of the vesicle is visible. Such portions may appear as small areas devoid of ribosomes, but may still be labeled with anti-GXM antibodies.