Figure 6.

Wnt5a can induce MMP-1 expression in endothelial cells. Adenoviral infected HMVEC to overexpress control LacZ, β-cateninS37A, or Wnt5a gene were used. (A) MMP-1 transcript levels analyzed by semiquantitative RT-PCR. β-actin expression was used to normalize samples. (B) Western blot analysis of secreted MMP-1 and band densitometry. Culture medium was collected 48 h after infection of HMVEC. α-tubulin Western blot was used to normalize protein load. (C) MMP-1 activity assay to quantify total secreted enzyme in culture medium from endothelial cells.