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Wnt5a induces expression of Tie-2 in HMVEC. Ectopic expression of control LacZ, β-cateninS37A, or Wnt5a gene was performed by adenoviral infections. (A) Tie-2 expression analysis by RT-PCR. β-actin was used to normalize samples. (B) Western blot analysis using an anti-Tie-2 antibody on plasma membrane proteins purified after surface biotinylation of cells and band densitometry. α-tubulin Western blot was performed from cell lysates to normalize protein content.
