Figure 2.

Micronuclear genome instability in TIF1-deficient T. thermophila. Ten clonal lines derived from the tif1-1::neo/TIF1 knockdown strain TXh48 were established and subjected to PCR analysis with primers sets that span sites for chromosome breakage sequence (CBS)-mediated chromosome fragmentation in the developing macronucleus. PCR primers derived from the right (R) and left (L) arms of all five micronuclear chromosomes were tested. 1–10, clonal TXh48 knockdown lines; WT, CU428. Early, ∼150 fissions after conferring resistance to high concentrations of pm (encoded by the tif1-1::neo disruption); late, ∼250 fissions later than “early.”