Table 2.
Chromosome transmission in TIF1 heterozygote and heterokaryon strains
| Cross | Strain | Phenotype | Expected % | Observed % |
|---|---|---|---|---|
| 1 | TXh102 × TXh106 | pms clonal progeny | 20a | 0, n = 48 |
| Sexual immaturity | 100 | 0, n = 48 | ||
| 2a | TXh102 × SB210 | 2dgalr/pmr progeny | 50 | 0, n = 40 |
| 2b | TXh106 × SB210 | 2dgalr/pmr progeny | 50 | 0, n = 40 |
Resistance to 2-deoxygalactose, cycloheximide, or paromomycin is encoded in the micronucleus of SB210 (gal1-1), SB1969 (chk1-1), and TIF1:tif1-1::neo heterozygotes, respectively. For cross 1, n is the number of clonal progeny tested for pm resistance. For cross 2, n is the number of small-scale matings in which the nonclonal progeny pool was tested for pm resistance.
a The predicted frequency for pms progeny is 25%. However, because the pairing efficiency in this cross was ∼80%, the expected frequency of pms clones is ∼20% (9.6 clones of 48 rather than 12 of 48).