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. 2006 Dec 1;116(12):3277–3291. doi: 10.1172/JCI29314

Figure 4. TSP-deficient thrombocytotic phenotype is transplantable.

Figure 4

(A–C) Lethally irradiated (9.5 Gy) WT control mice were transplanted with TSP-DKO bone marrow and TSP-DKO mice were transplanted with WT bone marrow. Ninety days after transplantation, platelet counts were 1.7 ± 0.1 × 106/μl in WT recipients transplanted with TSP-DKO bone marrow and 1.4 ± 0.6 106/μl in TSP-DKO recipients transplanted with WT bone marrow. P < 0.04. Platelet (A) and megakaryocyte levels (B) and vascular density (C) were determined in the transplanted mice. (D) When 5-FU was given (250 mg/kg i.v.) to the same mice, WT mice with TSP-deficient hematopoiesis displayed a faster platelet recovery than TSP-deficient mice that had been transplanted with WT bone marrow. On day 10, platelet levels were 0.18 ± 0.05 × 106/μl in TSP-DKO mice with WT hematopoiesis compared with 1.6 ± 0.27 × 106/μl in the WT recipients of TSP-deficient bone marrow (P < 0.03). Therefore, TSP from transplanted megakaryocytes, rather than TSP presented or secreted by nontransplanted bone marrow stromal components, may regulate megakaryopoiesis and thrombopoiesis following myelosuppression. *P < 0.05.