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. 2006 Nov 9;25(23):5504–5515. doi: 10.1038/sj.emboj.7601426

Figure 2.

Figure 2

Ska1 KT localisation requires KT–MT attachment at physiological temperatures. (A) HeLa S3 cells were either left untreated as a control or treated for 14 h with nocodazole and taxol, respectively. After PTEMF fixation, cells were stained with anti-Ska1 antibody (red), CREST serum (green) and DAPI (DNA, blue). (B) HeLa S3 cells were fixed with PTEMF either directly (control) or after incubation in ice-cold medium for another 2 h. Cells were stained with anti-Ska1 antibody (red), CREST serum (green) and DAPI (DNA, blue). (C) HeLa S3 cells were treated with nocodazole for 14 h and then either fixed directly or incubated in ice-cold medium for another 2 h in the presence of nocodazole. Cells were fixed and stained as in (B). (D) HeLa S3 cells were treated as in (C) but stained with anti-Mad2 antibody (red), CREST serum (green) and DAPI (DNA, blue). Scale bars=10 μm.