Skip to main content
. 2006 Nov 9;25(23):5504–5515. doi: 10.1038/sj.emboj.7601426

Figure 3.

Figure 3

Ska1 KT localisation requires the presence of Hec1 even in the cold. (A) HeLa S3 cells were treated for 48 h with control (GL2) and Hec1-specific siRNAs, respectively, then fixed with PTEMF and stained with anti-Ska1 (red), anti-Hec1 (green) and DAPI (DNA, blue). (B) HeLa S3 cells were treated for 48 h with control (GL2) and Hec1-specific siRNAs, respectively, before half of the samples were incubated for 2 h in ice-cold medium. Cells were fixed as in (A) but stained with anti-Ska1 antibody (red), CREST serum (green) and DAPI (DNA, blue). Scale bars=10 μm.