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. 2006 Nov 2;25(23):5443–5456. doi: 10.1038/sj.emboj.7601419

Figure 6.

Figure 6

Verapamil-induced redistribution of d/A from the enlargeosome lumen to the cytosol and vice versa. (A) Expression of both d/A and anx2 is unaffected by 16 h treatment of PC12-27 cells with 50 μM verapamil (verap), a blocker of the ABCB and ABCC transporters. (B) Untreated (NT) cells exhibiting the typical d/A punctate distribution coinciding with anx2 (left, see also Figures 3B and 4F), and diffuse to the cytosol in cells treated with verapamil as in (A). In contrast, the distribution of anx2 remains punctate (right). (C) Exocytic surface appearance of d/A induced by a 5-min stimulation with 5 μM ionomycin (left, see also Figures 1B and 4F, lower panel), no longer occurs in the cells treated with verapamil as in (A) (right). (D) Recovery of d/A, from the diffuse pattern in the cytosol induced by 16 h of verapamil (left) to the punctate distribution, already appreciable in cells washed and reincubated for 4 h (center), and complete in those reincubated for 24 h (right).