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. 2006 Sep 29;7(11):1111–1116. doi: 10.1038/sj.embor.7400818

Figure 2.

Figure 2

Effects on membrane insertion of single or pairs of Asn or Asp residues in the H2′ and H segments. The difference in the apparent free energy of insertion for constructs with and without Asn (N) or Asp (D) residues in H2′ (ΔΔGapp) for the different H segments is shown (see Table 1 for sequences). Black bars: 150-residue-long loop between the H2′ and H segments; white bars: 55-residue-long loop between the H2′ and H segments. The gel shows the two D3D constructs (H2′: 19L; H: 2D/12L/5A) and (H2′: 2D/17L; H: 2D/12L/5A), both with a short loop, translated in the presence of rough microsomes (unglycosylated, single glycosylated and double glycosylated forms of the protein are indicated by an open circle, one filled circle and two filled circles, respectively; the dye front is visible at the bottom of the gel). The corresponding ΔGapp values are 0.0 and −0.9 kcal/mol (Table 1), and ΔΔGapp=−0.9 kcal/mol (as shown by the white bar for the D3D pair in the bar graph). A, Ala, alanine; D, Asp, aspartic acid; L, Leu, leucine; N, Asn, asparagine.