Figure 5.

DNA strands of HJ are nicked symmetrically by rA22. A set of four HJs, each with a different 5′-³²P-end-labeled strand indicated in bold (0.1 to 0.14 pmol) was incubated with rA22 for 30 min and analyzed by electrophoresis on a denaturing polyacrylamide gel as in Fig. 3. Lanes 1, 2, 6, 10, and 14 show the mobilities of duplex or HJ probes without added proteins. The wedges above the other lanes indicate 0.34 to 3.4 μg of rA22 added to the reaction mixtures. Upper and lower arrows point to full-length and nicked strands, respectively.