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. Author manuscript; available in PMC: 2006 Dec 4.
Published in final edited form as: Alcohol Clin Exp Res. 2006 Jan;30(1):127–134. doi: 10.1111/j.1530-0277.2006.00008.x

Fig. 5.

Fig. 5

Heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) amelioration of embryonic cell death induced by exposure to ethanol in vitro. Intact visceral yolk sacs were isolated from nontreated dams on E7.5 and cultured for 1 or 3 hour in Ham’s F10 medium alone (control), medium containing 400 mg% ethanol (EtOH), or medium containing 400 mg% ethanol and 5 nM HB-EGF (EtOH+HB-EGF). The embryonic portion of the yolk sacs was then isolated, fixed, permeabilized, and assayed for terminal deoxynucleotidyl transferase-mediated dUTP-X nick end labeling (TUNEL) by the fluorescence microscopy procedure detailed in the Materials and Methods section. Mean and SE are shown. N = 4 to 5 embryos per treatment. Bars labeled with nonmatching letters were significantly different.