Figure 5. E2F3 overexpression in MCF7 cells allows bypass of antimitogenic checkpoints.
(A) MCF7 cells infected with adenoviral vectors encoding either lacZ or E2F3 were harvested 3 days after infection, lysed, separated by SDS-PAGE, and immunoblotted for determination of E2F3, RB, MCM7, and PCNA expression levels. Cdk4 served as a loading control. (B) The adenovirus-infected cells represented in A were cultured in media containing FBS, CDT, CDT/Tam, or CDT/ICI for 3 days or were treated as described above with 16 μM CDDP or 5 Gy IR prior to BrdU labeling for 10 hours. Cells were then fixed, and BrdU immunofluorescence and scoring were performed.