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Effect of introducing a discontinuity on expression and activity. Time courses of lactose transport and levels of membrane expression of mutants Δ5 and Δ5-split (A), Δ12 and Δ12-split (B), and Δ20 and Δ20-split (C). E. coli T184 harboring a plasmid encoding a given mutant or its derivative split construct were assayed for lactose transport (Upper) and membrane expression of the permease (Lower) as described in the text.
