Skip to main content
. 2000 Jul 4;97(16):8938–8943. doi: 10.1073/pnas.140224497

Table 1.

Genotypes and phenotypes of the mutants used in this study

Mutant Phenotype Sequence
WT Red 189TDAPSSATVANAVGANHSAFSL––––––––––––––––––––––KLALELFRQPK221
Δ5 Red 189TDAPSSATVANAVGANH–––––––––––––––––––––––––––KLALELFRQPK221
Δ5-KasI Red 189TDAPSSATVANAVGANH–––––––––––––––GA––––––––––KLALELFRQPK221
Δ5-split Red 189TDAPSSATVANAVGANH*–––––––––––––––––––––––––MKLALELFRQPK221
Δ5 + 12 Hphil 1 Red 190TDAPSSATVANAVGANH––––––––––GAEDEDCPEDEHCA–––KLALELFRQPK221
Δ5 + 12 Hphil 2 Red 190TDAPSSATVANAVGANH––––––––––GAEDEDEDEDEDCA–––KLALELFRQPK221
Δ5 + 12 Hphob 1 Halo 190TDAPSSATVANAVGANH––––––––––GAVLIFWAIFVSCA–––KLALELFRQPK221
Δ5 + 12 Hphob 2 Halo 190TDAPSSATVANAVGANH––––––––––GAIVIVIVIVIVCA–––KLALELFRQPK221
Δ12 Red 189TDAPSSATVA––––––––––––––––––––––––––––––––––KLALELFRQPK221
Δ12-KasI Red 189TDAPSSATVA––––––––––––––––––––––GA –––––––––KLALELFRQPK221
Δ12-split Red 189TDAPSSATVA*––––––––––––––––––––––––––––––––MKLALELFRQPK221
Δ12 + 12 Hphil 1 Red 190TDAPSSATVA–––––––––––––––––GAEDEDCPEDEHCA–––KLALELFRQPK221
Δ12 + 12 Hphil 2 Red 190TDAPSSATVA–––––––––––––––––GAEDEDEDEDEDCA–––KLALELFRQPK221
Δ12 + 12 Hphob 1 White 190TDAPSSATVA–––––––––––––––––GAVLIFWAIFVSCA–––KLALELFRQPK221
Δ12 + 12 Hphob 2 White 190TDAPSSATVA–––––––––––––––––GAIVIVIVIVIVCA–––KLALELFRQPK221
Δ20 White 189TDAPSS––––––––––––––––––––––––––––––––––––––––––ELFRQPK221
Δ20-KasI White 189TDAPSS––––––––––––––––GA –––––––––––––––––––––––ELFRQPK221
Δ20-split Red 189TDAPSS*––––––––––––––––––––––––––––––––––––––––MELFRQPK221
Δ20 + 22 Hphil Red 190TDAPSS–––––––––GATSTATSTSCATSTATSTSCA––––––––––ELFRQPK221
Δ20 + 29 Hphil Red 190TDAPSS–––––––GAGGGGGGGGAGGGGGGGGGAGGGGGGGGGA––––ELFRQPK221
Δ20 + 40 Hphil Red 190TDAPSSGATSTATSTSCATSTATSTSCATSTATSTSCATSTATSTSCAELFRQPK221

Phenotype refers to color on MacConkey lactose indicator plates. Asterisks indicate a stop codon, and the italicized G and A indicate the amino acids glycine and alanine encoded by the unique KasI restriction enzyme site.