Table 1.
The shotgun scanning code
| Amino acid | Shotgun codon* | Shotgun substitutions |
|---|---|---|
| C | KST | A/C/G/S |
| D | GMT | A/D |
| E | GMA | A/E |
| F | KYT | A/F/S/V |
| G | GST | A/G |
| H | SMT | A/H/D/P |
| I | RYT | A/I/T/V |
| K | RMA | A/K/E/T |
| L | SYT | A/L/P/V |
| M | RYG | A/M/T/V |
| N | RMC | A/N/D/T |
| P | SCA | A/P |
| Q | SMA | A/Q/E/P |
| R | SST | A/R/G/P |
| S | KCC | A/S |
| T | RCT | A/T |
| V | GYT | A/V |
| W | KSG | A/W/G/S |
| Y | KMT | A/Y/D/S |
For each amino acid, the appropriate shotgun codon ideally encodes only the wt amino acid or alanine, but the nature of the genetic code necessitates the occurrence of two other amino acids for some shotgun substitutions. Single-letter amino acid and nucleotide abbreviations are used.
*
DNA degeneracies are represented by IUB code (K = G/T, M = A/C, N = A/C/G/T, R = A/G, S = G/C, W = A/T, Y = C/T).