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. Author manuscript; available in PMC: 2006 Dec 4.
Published in final edited form as: Biochemistry. 2003 May 20;42(19):5850–5856. doi: 10.1021/bi034061w

Figure 2.

Figure 2

Reduction of wild-type nitroalkane oxidase by nitroethane. NAO (final concentration of 14 μM) was rapidly mixed with nitroethane (final concentration of 58 mM) under anaerobic conditions. The reaction was monitored first at multiple wavelengths (A) and then specifically at 450 nm (B). The line in panel B is a fit of the data to eq 2, with every 10th data point shown. Panel C shows the residuals from a fit of the data to eq 2 using three (—) or two (– – –) phases. Conditions were 100 mM Hepes, pH 8.0, and 30 °C.