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Reduction of wild-type nitroalkane oxidase by 1-nitrobutane. NAO (final concentration of 30 μM) was rapidly mixed with 1-nitrobutane (final concentration of 10 mM) under anaerobic conditions, and the reaction was monitored at 450 nm. The line is a fit of the data to eq 2 containing only two phases, with every 10th data point shown. The initial absorbance of the oxidized enzyme is indicated by the arrow. Conditions as described in the legend of Figure 2.
