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. Author manuscript; available in PMC: 2006 Dec 4.
Published in final edited form as: Biochemistry. 2003 May 20;42(19):5850–5856. doi: 10.1021/bi034061w

Figure 5.

Figure 5

Concentration dependence of reduction of D402E nitroalkane oxidase by 1-nitrobutane. The mutant enzyme (final concentration of 18 μM) was rapidly mixed with the indicated concentrations of 1-nitrobutane (●) or 1-[1,1-2H2]nitrobutane (○) under anaerobic conditions, and the reaction was monitored at 450 nm. Conditions were 100 mM Hepes, pH 8.0, and 5 °C. The error in each data point is smaller than the symbol.