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. Author manuscript; available in PMC: 2006 Dec 4.
Published in final edited form as: Biochemistry. 2003 May 20;42(19):5850–5856. doi: 10.1021/bi034061w

Table 2.

Steady State Kinetic Isotope Effects for Nitroalkane Oxidasea

wild type
D402E
DVmax D(V/K) DVmax D(V/K)
nitroethane 1.4 ± 0.2 9.2 ± 1.1 3.6 ± 0.7 9.6 ± 2.3
1-nitrobutane 1.04 ± 0.09 1.7 ± 0.6 1.17 ± 0.05 6.8 ± 1.0
a

Conditions as described in footnote a of Table 1.