Table 1.
Thermodynamic parameters in the interaction between TRAF2 and receptor peptides as measured by ITC
| Receptor peptide | Sequence | T, °C | Ka, 103⋅M−1 | Kd, mM | ΔG, kcal/mol | ΔH, kcal/mol | −TΔS, kcal/mol | N | Ligand, mM | Protein, mM |
|---|---|---|---|---|---|---|---|---|---|---|
| hCD30 (573–583) | SDVMLSVEEEG | 20 | 28.5 ± 3.5 | 0.04 | −5.97 | −14.0 ± 0.8 | 8.03 | 0.86 ± 0.05 | 6.7 | 0.056 |
| hCD40 (250–266) | PVQETLHGCQPVTQEDG | 20 | 15.9 ± 1.5 | 0.06 | −5.63 | −9.5 ± 1.0 | 3.87 | 1.1 ± 0.1 | 5.9 | 0.056 |
| hOX40 (262–266) | PIQEE | 20 | 20.8 ± 2.5 | 0.05 | −5.78 | −13.0 ± 0.9 | 7.22 | 0.86 ± 0.07 | 12.9 | 0.056 |
| hTNFR2 (420–428) | QVPFSKEEC | 20 | 1.76 ± 0.04– | 0.5* | −4.35 | ND | ND | ND | 9.7 | 0.16 |
| 2.0 ± 0.05* | ||||||||||
| m4-1BB (231–236) | GAAQEE | 20 | 0.91 ± 0.014– | 1.0* | −3.96 | ND | ND | ND | 13.4 | 0.16 |
| 1.11 ± 0.011* | ||||||||||
| hLMP1 (204–210) | PQQATDD | 20 | 0.50 ± 0.017– | 1.9* | −3.62 | ND | ND | ND | 17.2 | 0.16 |
| 0.55 ± 0.019* | ||||||||||
| hCD30 (573–583) | SDVMLSVEEEG | 10 | 36.0 ± 5.0 | 0.03 | −5.90 | −11.5 ± 0.5 | 5.60 | 0.63 ± 0.02 | 6.7 | 0.28 |
| hCD30 (573–583) | SDVMLSVEEEG | 20 | 23.7 ± 2.0 | 0.04 | −5.86 | −14.9 ± 0.7 | 9.04 | 0.63 ± 0.02 | 6.7 | 0.28 |
| hCD30 (573–583) | SDVMLSVEEEG | 30 | 14.4 ± 0.4 | 0.07 | −5.76 | −16.4 ± 0.2 | 10.64 | 0.62 ± 0.006 | 6.7 | 0.28 |
Ka, association constant; Kd, dissociation constant; h, human; ND, not determined.
*
The ranges of Ka for these three receptor peptides were determined at N = 0.5 and N = 1.5. The corresponding Kd is calculated from the median value of the Ka range.