Skip to main content
. 2000 Jul 18;97(16):8967–8972. doi: 10.1073/pnas.150236097

Figure 1.

Figure 1

Preparative affinity purification of U2 snRNPs from T. brucei. U2 snRNPs were affinity purified from T. brucei extract, separated on a preparative SDS/polyacrylamide gel (15%), transferred to nitrocellulose, and visualized by Ponceau S staining. The identification of U2-specific and common proteins is indicated on the right (compare with ref. 8), molecular mass markers on both sides (sizes in kDa). For microsequencing, the four common proteins 8.5 kDa, 10 kDa, 12.5 kDa, and 14 kDa were selected, and in addition, the region around 15 kDa containing both common and U2-specific proteins (15K-mix).