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. 2000 Jul 18;97(16):8967–8972. doi: 10.1073/pnas.150236097

Figure 5.

Figure 5

The trypanosomal 14-kDa protein complements specifically a yeast SMG disruption. A haploid yeast strain carrying a chromosomal disruption of the SMG gene complemented by a wild-type copy of the gene on a URA3-marked plasmid was transformed with LEU2-marked plasmids expressing the various trypanosomal proteins or, as a positive control, the yeast SmG protein in a LEU2-marked plasmid. As a negative control, the same strain was transformed with the expression vector only. Transformants were then subcloned on complete media (Right) or on selective media (Center) that contains FOA, a drug preventing growth of cells expressing the URA3 marker. A wild-type ura3− strain was used as a positive control for FOA selection. (Left) Relevant characteristics of the strains present in the different sectors. The various strains grew equally well on complete media (Right). Cells expressing the trypanosomal 14-kDa protein were able to grow on selective media, indicating that it complements the SMG gene disruption.