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Proteasome inhibitor MG132 blocks the T3-induced degradation of TRβ1. Cellular lysates (75 μg) from CV1 cells transfected with TRβ1 expression plasmid that were treated with (lanes 4 and 5) or without (lanes 2 and 3) MG132 (50 μM for 12 h) were analyzed by Western blotting similarly as described for Fig. 2A. For control, lane 1 is the marker TRβ1 from the in vitro translation.
