Figure 3.

Complex I contains three DNA molecules. BAF was bound to short, long, or a mixture of long and short duplex oligonucleotides as indicated. The short oligonucleotide is the substrate A shown in Fig. 1. The long oligonucleotide is the same duplex as substrate A with a 20-base, single-strand tail added to retard its electrophoretic mobility. An asterisk indicates that the DNA is labeled with ³²P. The gel was electrophoresed for an extended period to resolve the multiple species of complex I with different electrophoretic mobilities. The presence of both short and long DNA results in the splitting of complex I into four bands. We interpret this to indicate the presence of three DNA molecules in complex I, with all four possible combinations of short and long DNA molecules.