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. 2000 Jul 18;97(16):9003–9008. doi: 10.1073/pnas.160004997

Figure 2.

Figure 2

Analysis of TFO-induced recombinant clones expressing wild-type (WT) TK. (A) Possible pathways to generate a wild-type TK gene from the tandem mutant TK genes in FL-10 cells. The diagram illustrates crossover recombination, yielding (via multiple crossovers) a single wild-type TK gene in a nonconservative event, and gene conversion, in which there is information transfer from one TK gene to the other to correct the XhoI linker insertion mutation, with retention of both TK genes in a conservative event. (B) Expected pattern of bands on Southern analysis of parental and recombinant HAT-resistant clones, depending on the nature of the recombination event. The hypothetical band pattern is based on the indicated restriction digestion of genomic DNA and hybridization with a TK gene fragment as a probe. (C) Southern blot analysis of genomic DNA from the parental FL-10 cells (P) and seven HAT-resistant clones produced by TFO microinjection (lanes 1–7). The samples were restricted with the indicated enzymes, and the Southern hybridization was performed with the 2.5-kb TK gene BamHI fragment as a probe.