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The effect of the iscS mutation on the activity of FNR mutants that exhibit altered Fe-S cluster requirements. β-galactosidase activity produced from the dmsA′∷lacZ promoter fusion was assayed from strains (IscS+; PK3293, filled columns, IscS-; PK4359, open columns) grown under aerobic conditions (Upper) and anaerobic conditions (Lower). WT FNR, FNR-D154A, and FNR-L28H were present on the multicopy plasmid pACYC184 (58).
