Figure 1.

Element decompression and part design. (A) The coding regions of genes 2.8 and 3 overlap in the wild-type T7 genome. The RBS of gene 3 (underlined) is encoded within gene 2.8. (B) Distinct genetic parts make up the T7.1 genome. The natural RBS and start codon (green) for gene 3 are disrupted by point mutations (capitals); mutations do not change the amino-acid sequence of the 2.8 protein. Parts 28 and 29 are separated by bracketing restriction sites, BamHI (blue) and EagI (orange). Supplementary Figure S3 lists all changes in the DNA sequence of T7.1 relative to wild-type T7.