. 2006 Jan 31;2:2006.0004. doi: 10.1038/msb4100046

Table 3.

Estimated biomass composition of M. barkeri

Metabolite	mmol gDW⁻¹	Metabolite	mmol gDW⁻¹	Metabolite	mmol gDW⁻¹
Protein (63%) ^a		RNA (24%) ^a		Soluble pools (4%)
Alanine	0.5621	ATP	0.1846	Putrescine	0.0262
Arginine	0.3237	CTP	0.1379	Homospermidine	0.0047
Asparagine	0.2638	GTP	0.2222	Acetyl-Coenzyme A	0.0001
Aspartate	0.2638	UTP	0.1489	Coenzyme A	<0.0001
Cysteine	0.1002	DNA (4%) ^a		NAD⁺	0.0022
Glutamine	0.2880	dATP	0.0331	NADH	0.0001
Glutamate	0.2880	dCTP	0.0215	NADP⁺	0.0001
Glycine	0.6704	dGTP	0.0215	NADPH	0.0004
Histidine	0.1037	dTTP	0.0331	Succinyl-Coenzyme A	<0.0001
Isoleucine	0.3179	Lipid (5%)		Coenzyme M	0.0206
Leucine	0.4930	Archaetidylglycerol	0.0007	Coenzyme F420	0.0008
Lysine	0.3755	Hydroxyarchaetidylglycerol	0.0101	Tetrahydrosarcinapterin	0.0236
Methionine	0.1682	Archaetidylinositol	0.0027	Adenosylcobalamin-HBI	0.0047
Phenylalanine	0.2027	Hydroxyarchaetidylinositol	0.0135	Coenzyme F430	0.0020
Proline	0.2419	Archaetidylethanolamine	0.0007	Coenzyme B	0.0005
Serine	0.2361	Hydroxyarchaetidylethanolamine	0.0027	5,6,7,8-Tetrahydrofolate	0.0001
Threonine	0.2776	Archaetidylserine	0.0013	Coenzyme F390	<0.0001
Tryptophan	0.0622	Hydroxyarchaetidylserine	0.0115	ATP	0.0040
Tyrosine	0.1509	Glucosaminyl archaetidylinositol	0.0162	ADP	0.0020
Valine	0.4631	Carbohydrates (<1%)		AMP	0.0010
		Glycogen	0.0154

^aRelative ratios of wt% of protein, RNA and DNA were estimated from the composition of a typical prokaryotic cell (Neidhardt et al, 1990). The values were taken from published data and converted to mmol gDW⁻¹ and the wt% of each category is given (for references, see Supplementary information 1).