Table 1.
Interacting pairs of synthetic shuffled DZF domains identified by genetic selection
| Zif268 hybrids | RNAP α hybrids | Frequency |
|---|---|---|
| Library set A | ||
| Ik-Eo-Eo | Eo-Ik-Ik | 2 |
| Ik-Eo-Eo | Eo-Eo-Ik | 1 |
| Ik-Eo-Eo | Ik-Eo-Eo | 4 |
| Ik-Eo-Eo | Ik-Ik-Eo | 1 |
| Ik-Ik-Eo | Eo-Ik-Ik | 2 |
| Ik-Ik-Eo | Eo-Eo-Ik | 3 |
| Ik-Ik-Eo | Ik-Ik-Eo | 1 |
| Ik-Ik-Eo | Ik-Eo-Eo | 1 |
| Ik-Ik-Eo | Pe-Eo-Eo | 2 |
| Ik-Ik-Eo | Pe-Ik-Ik | 1 |
| Ik-Eo-Eo | Pe-Eo-Eo | 1 |
| Ik-Eo-Eo | Eo-Pe-Pe | 1 |
| Pe-Eo-Eo | Ik-Ik-Eo | 3 |
| Pe-Pe-Hd | Hd-Hd-Pe | 4 |
| Pe-Hd-Hd | Hl-Eo-Eo | 1 |
| Ik-Ik-Hd | Pe-Pe-Eo | 1 |
| Pe-Pe-Eo | Eo-Eo-Hd | 2 |
| Ik-Ik-Hd | Pe-Pe-Eo | 1 |
| Library set B | ||
| Hl-Hd-Hd | Hl-Hd-Hd | 2 |
| Hd-Hl-Hl | Hd-Hl-Hl | 1 |
| Pe-Hd-Hd | Hl-Hd-Hd | 2 |
| Pe-Pe-Hd | Hl-Hl-Hd | 2 |
| Pe-Pe-Hd | Hd-Pe-Pe | 4 |
| Pe-Hd-Hd | Hd-Hd-Hl | 1 |
| Pe-Pe-Hd | Hd-Hd-Pe | 6 |
| Library set C | ||
| Tr-Eo-Eo | Eo-Eo-Hd | 3 |
| Identities of the amino-terminal C2H2 ZF, the interfinger linker, and the carboxy-terminal C2H2 ZF (shown from left to right) for each of the synthetic DZFs identified from the three different selection ‘sets' A, B, and C are shown. Abbreviations describing the parental source of fingers and linkers are as defined in the legend to Figure 2. Location of the synthetic DZF on the Zif268 or RNAP α-subunit and the number of times each pair of DZFs were isolated are shown. Four DZF pairs chosen for additional analysis are in bold. | ||