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. 2006 Jun 6;2:2006.0029. doi: 10.1038/msb4100067

Figure 3.

Figure 3

Biochemical validation of target genes. Results of RT–PCR analysis in NIH3T3 fibroblasts expressing an inducible ERE2F1 or a transactivation-deficient mutant, MERE2F1. At time zero, medium containing 500 nM of 4-hydroxytamoxifen (OHT) was added and transcript levels of (A) DLG7 and (B) CDC16 were determined using gene-specific primers at the indicated times by RT–PCR. The respective transcript levels with 10 μg/ml of cyclohexamide (CTX) added are shown in (C) DLG7 and (D) CDC16. GAPDH was used as a standardization control. Plotted data reflect results after normalization with GAPDH.