Figure 3.

Ranges of Gibbs energies of reaction with and without considering the respective reaction's operation in the metabolic network. White bars indicate possible ranges due to the provided concentration ranges (see Materials and methods), whereas the black bars display thermodynamically feasible ranges after introducing the constraints of the reaction network. Abbreviations: me(NADH), malic enzyme (NADH dependent); pdh, pyruvate dehydrogenase; nadk, NAD kinase; akgdh, α-ketoglutarate dehydrogenase; cs, citrate synthase; udh, cytosolic transhydrogenase; atps, ATPase; fba, fructosebisphosphate aldolase; me(NADPH), malic enzyme (NADPH dependent); mdh, malate dehydrogenase; rpi, ribosephosphate isomerase; pgk, phosphoglycerate kinase; gapd, glyceraldehyde 3-phosphate dehydrogenase; tpi, triosephosphate isomerase; pgm, phosphoglycerate mutase; eno, enolase.