Figure 6.
Intracellular concentration of glycolytic and TCA cycle intermediates following 5.6 mM glucose pulse, expressed in micromoles per gram of dry weight (μmol/g DW) except mentioned otherwise. (A) Glucose-6-phosphate (G6P), (B) fructose-6-phosphate (F6P), (C) fructose-1,6-biphosphate (F1,6P2), (D) fructose-2,6-biphosphate (F2,6P2) expressed as normalized to the steady-state value (see Materials and methods), (E) 2 and 3 phosphoglycerate (2PG+3PG), (F) phosphoenolpyruvate (PEP), (G) 6-phosphogluconate (6PG), (H) glucose-1-phosphate (G1P), (I) trehalose-6-phosphate (T6P), (J) citrate (CIT), (K) α-keto-glutarate (αKG), (L) succinate (SUC), (M) fumarate (FUM), (N) malate (MAL) and (O) NADH/NAD ratio expressed as normalized to the steady-state value. The data plotted originate from at least two independent pulse experiments.