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Different patterns of KIP binding during cyclin D1–cdk4 and cyclin E–cdk2 activation. Cdk4 (A) and cyclin E (C) immunoprecipitates (IP) from cell lysates recovered at intervals after readdition of estradiol to steroid-depleted MCF-7 cells were resolved and analyzed by immunoblotting with the indicated antibodies. Cyclin D1 (B) and cyclin E (D) immunoprecipitates were assayed for kinase activity (36) at intervals after estradiol stimulation of quiescent MCF-7.
