Table 1.

Interaction between Ras/Rap1 and AF-6

LBD fusion	β-Galactosidase activity of GAD-fused AF-6 domain
	AF-6N	AF-6-RBD1	AF-6-RBD2
RasV12	99.0  ± 1.6	210.0  ± 1.8	0.7  ± 1.4
RasN17	0.6  ± 1.4	0.9  ± 1.4	0.6  ± 1.1
RapE63	120.0  ± 1.7	350.0  ± 1.9	1.2  ± 1.1
RapN17	0.7  ± 1.7	0.8  ± 1.9	0.9  ± 1.1
Lamin	0.7  ± 1.3	0.8  ± 1.1	0.7  ± 1.5

Interaction between Ras/Rap1 and AF-6. The AF-6N, AF-6-RBD1, and AF-6-RBD2 GAD fusion products were transformed individually into the yeast reporter strain L40 with LBD fusions containing constitutively active and negative mutant forms of Ha-Ras and Rap1, as well as lamin as a negative control. Transformants were grown in selective synthetic medium, and βgalactosidase activity was assayed with O-nitrophenyl-β-galactosidase; values (miller units) are the means ± SD of triplicate determinations.