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Nip3 mRNA levels are induced in response to hypoxia in several different cell lines. Cells were incubated under normoxic (20% O2) or hypoxic (0.5% O2) conditions for 15 h. Radiolabeled probes specific for Nip3, Glut-1, or 28S rRNA were used to visualize mRNAs from total RNA by Northern analysis.
