Table 1.
gd RNA, mg/ml | Number of cuticles | % Partially rescued | % Rescued to wild type | % Ventralized |
---|---|---|---|---|
0.03 | 91 | 31 | 66 | 3 |
0.15 | 40 | 38 | 20 | 42 |
0.75 | 52 | 6 | 2 | 92 |
1.50 | 64 | 0 | 0 | 100 |
Embryos from gd7/gd7 mothers were injected with gd RNA at the indicated concentrations, and cuticles produced by injected embryos were examined. Partial rescue includes cuticles having the dorsolateral Filzkörper (arrow in Fig. 2D) or the Filzkörper and some ventral denticles (inverted v in Fig. 2D). Wild type indicates embryos that hatched out of the eggshell (Fig. 2D) or unhatched embryos having a wild-type cuticle pattern. Ventralized represents cuticles having ventral denticles but no Filzkörper (Fig. 2F), which were produced by embryos having a ventralized phenotype at the gastrulation stage (Fig. 2E).