Table 2.

The gastrulation patterns of embryos injected with wild-type or mutant gd RNA

Recipient  embryos	gd RNA	Number of embryos	Dorsalized, %	Ventralized, %	Lateralized, %
Wild type	Wild type	63	0	100	0
nudel	Wild type	112	77	3	20
pipe	Wild type	116	16	0	84
windbeutel	Wild type	79	4	77	19
snake	Wild type	75	100	0	0
gd	S-A	324	100	0	0
gd	D-N	267	100	0	0

Wild-type embryos or embryos lacking maternal activity of indicated genes (genotypes are given in Materials and Methods) were injected with ≥0.75 mg/ml gd RNA encoding wild-type protein or a point mutant (S-A and D-N), and the response of the injected embryos was scored at the gastrulation stage. The gastrulation phenotypes of dorsalized, ventralized, and lateralized are shown in Fig. 2 A, E, and G, respectively. Uninjected gd mutant embryos are 100% dorsalized at gastrulation.