Table 2.

Expression of Tn5lacZ fusions in developing cells

Strain	Expression		β-galactosidase specific activity*		Activity ratio§
	Start†	C-depend‡	Fruiting bodies	Peripheral rods
pilA	0	−	600  ± 52	512  ± 43	1.2
Ω4521	2	−	632  ± 27	525  ± 79	1.2
Ω4455	3	−	160  ± 16	152  ± 18	1.0
Ω4469	4	−	647  ± 58	800  ± 85	0.8
Ω4414 (dev)	6	+	470  ± 48	107  ± 36	4.4
Ω7536	8	+	347  ± 55	24  ± 2	14.4
Ω4403	11	+	323  ± 14	66  ± 23	4.9
Ω4459	15	+	295  ± 14	34  ± 11	8.7
Ω4435	20	+	170  ± 40	48  ± 5	3.5

^*β-galactosidase specific activity for separated fruiting body cells and peripheral rods carrying the indicated Tn5lacZ fusions (15) was determined after 24 h of development on TPM starvation agar. Mean specific activity ± SD were determined from three independent experiments. 

^†Hours after the initiation of development by starvation when each reporter starts its expression. 

^‡+ indicates that expression depends on C-signaling, and − indicates that it does not (33). 

^§β-galactosidase specific activity in fruiting bodies divided by that in peripheral rods.