Table 3.

Sporulation of dev mutants in submerged culture

Strain	Percent wild-type sporulation
	Morphological spores*	Viable spores†
DK1622	100.0  ± (2.9)	100.0  ± (3.4)
DK4473	12.6  ± (3.0)	5.4  ± (2.1)
DK5508	28.0  ± (1.3)	0.2  ± (0.2)
DK11239	20.7  ± (1.1)	1.3  ± (0.4)

Development in submerged culture for 5 days. Each assay was made three times. The mean values for three independent assays with the standard deviations in parentheses are shown. 

^*Values were determined by counting the number of sonication-resistant, spherical-shaped cells in a Petroff-Hausser chamber observed by phase-control microscopy. 

^† Viable spore assay is described in Materials and Methods.