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One-dimensional isoelectricfocusing analysis of 35S-methionine-labeled cellular lysates precipitated with control IgG, W6/32, or 25D3 mAbs. 25D3 precipitates Mamu-AG-specific bands (four lower arrows) from trophoblasts and Mamu-AG transfectants, but not from PBLs or B*01 transfectants.
