Figure 4.

Vector expression in mice transplanted with transduced marrow. Marrow was transduced with either the uninsulated vector MGPN2 or the insulated vector INS4(+) and was used to transplant syngeneic recipients following 1025 cGy whole body irradiation. (a) At the indicated months posttransplant, blood samples were collected and the percentage of white blood cells (WBC) expressing GFP was determined by flow cytometry as described in Fig. 2a. (b) Likewise, marrow aspirates were collected at the indicated months posttransplant by survival surgery and the percentage of progenitors [colony-forming cells (CFC)] expressing Neo was determined by colony formation under G418 selection as described in Fig. 2a. Data for vector MGPN2 include eight mice from three separate experiments, and data for vector INS4(+) include nine mice from three separate experiments. Only those mice with detectable provirus at 8 months posttransplant were included in the analysis.