Table 2.
Vector | Replicates | Neo expression
|
GFP expression
|
||||
---|---|---|---|---|---|---|---|
Unselected
|
G418-selected
|
||||||
G418r* | P† | % GFP+‡ | P | % GFP+ | P | ||
MGPN2 | 4 | 53 ± 9 | 18 ± 14 | 47 ± 17 | |||
INS4(+) | 4 | 77 ± 9 | 0.03 | 46 ± 24 | 0.02 | 55 ± 26 | 0.25 |
INS4(−) | 3 | 51 ± 11 | 0.86 | 21 ± 21 | 0.16 | 30 ± 28 | 0.08 |
Percent G418r colonies = [colony count with G418]/[colony count without G418] × 100.
Probability compared to MGPN2 control using t test.
Percent GFP+ cells determined by flow cytometric analysis of colony pools.