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. 2000 Jul 25;97(16):9156–9161. doi: 10.1073/pnas.160213497

Figure 2.

Figure 2

Specific interaction between BCMA and TALL-1. (a) BCMA interacts with sTALL-1 but not sTRAIL. 293 cells were transfected with pCMV-BCMA-HA (lanes 1–4, 7, and 8) or an empty control plasmid (lanes 5 and 6). Twenty-four hours after transfection, cells were lysed and the lysates were mixed with control medium (lanes 1 and 2) or conditioned medium containing Flag-sTALL-1 (lanes 3–6) or Flag-sTRAIL (lanes 7 and 8). The mixtures were immunoprecipitated with a monoclonal anti-HA antibody (lanes 2, 4, 6, and 8) or mouse control IgG (lanes 1, 3, 5, and 7). The immunoprecipitates were analyzed by Western blot with anti-Flag antibody (Upper). The same blot was stripped and reprobed with anti-HA antibody (Lower). The higher-molecular-weight band is probably a glycosylated form of BCMA, which has been shown to be a glycoprotein (25). (b) sTALL-1 does not bind to TRAIL receptors. 293 cells were transfected with secretion expression plasmids for Flag-Fc, Flag-sTRAIL-R1-Fc, and Flag-sTRAIL-R2-Fc. Twenty-four hours after transfection, aliquots of the cell culture medium were mixed with conditioned medium containing either Flag-sTALL-1 (lanes 1–3) or Flag-sTRAIL (lanes 4–6). The mixtures were precipitated with GammaBind G Sepharose beads. Proteins bound to the beads were analyzed by Western blot with anti-Flag antibody.