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. 2000 Jul 25;97(16):9156–9161. doi: 10.1073/pnas.160213497

Figure 3.

Figure 3

Flow cytometry analysis of sTALL-1 binding to BCMA. (a) TALL-1 receptor expression in various cell lines detected by Flag-sTALL-1. The indicated cells were incubated with control medium (shaded histograms) or Flag-sTALL-1 containing medium (solid-line histograms). Cells were washed and analyzed by flow cytometry with a monoclonal anti-Flag antibody. (b) BCMA is targeted to plasma membrane, where it can bind to sBCMA. 293 cells were transfected with the indicated expression plasmids. Twenty-four hours after transfection, cells were incubated with control medium (shaded histograms) or Flag-sTALL-1 containing medium (solid-line histograms). Flow cytometry analysis of the transfected intact cells was performed with anti-Flag antibody. (c) sBCMA blocks sTALL-1 binding to its receptor. Bjab cells were incubated with control medium (shaded histogram), Flag-sTALL-1 containing medium + control medium (solid-line histogram), or Flag-sTALL-1 containing medium + Flag-sBCMA-Fc containing medium (dashed-line histogram). The treated cells were analyzed by flow cytometry with anti-Flag antibody. Similar data have been obtained for the above flow cytometry analysis with purified Flag-sTALL-1.