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American Journal of Human Genetics logoLink to American Journal of Human Genetics
. 1990 Sep;47(3):515–523.

HLA-DQα allele and genotype frequencies in various human populations determined by using enzymatic amplification and oligonucleotide probes

Rhea Helmuth, Nicola Fildes, Edward Blake, Michael C Luce, J Chimera, Roberta Madej, C Gorodezky, Mark Stoneking, Norma Schmill, William Klitz, Russell Higuchi, Henry A Erlich
PMCID: PMC1683881  PMID: 2393024

Abstract

Allele and genotype frequencies at the HLA-DQα locus have been determined by the use of polymerase chain reaction (PCR) amplification and nonradioactive oligonucleotide probes. The probes define six alleles and 21 genotypes in a dot-blot format. A total of over 1,400 individuals from 11 populations has been typed by two different laboratories using this method. In contrast to some variable-number-of-tandem-repeat markers that have been used for identity determination, DQα genotype frequencies do not deviate significantly from Hardy-Weinberg equilibrium in all populations studied. The distribution of alleles varies significantly between most of these populations. In Caucasians, the allele frequencies range from 4.3% to 28.5%. In this population, the power of discrimination is .94, and, for paternity determination, the power of exclusion is .642. These population data will allow the use of the HLA-DQα marker in paternity determination, the analysis of individual identity in forensic samples, and anthropological studies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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