Figure 2.
Analysis of HIV DNA accumulation in HIV-1BaL infected MDM in the presence or absence of MDC (240 ng/ml) (A) or 3′-azido3′-deoxythimidine (AZT) (10 μM) (B). In contrast to the inhibitory effects exerted by MDC on HIV replication in MDM of two independent donors, as determined by supernatant-associated RT activity, a faithful indicator of virion production (45), no substantial effects were observed in terms of accumulation of HIV DNA up to 72 h postinfection, as quantified by a TaqMan assay (Applied Biosystems) (25). n.d., not done.