Figure 1.

(a) Partial karyotype showing the normal (outside) and translocated (inside) homologues of chromosomes 5 and 11. (b) Schematic presentation of the translocation t(5;11)(q31;q23). (c) As a result of the translocation the MLL-yeast artificial chromosome clone (red) is split and partly translocated to chromosome 5 (green painting probe), thus generating signals on both chromosomes 11 as well as on the affected chromosome 5. (d) G-banding of a representative metaphase from the leukemic clone. (e and f) Hybridization of a 1,176-bp clone of the human GRAF gene onto a representative normal metaphase chromosome confirms its location at 5(q31).